Mammalian Two-hybrid for Protein-Protein Interaction Analysis
Protein-protein interactions, commonly known as PPIs, are of utmost importance in various biological processes, including but not limited to signal transduction, gene expression, and metabolic pathways. The complexity and sheer significance of PPIs require us to comprehend them in their entirety to unravel the mechanisms underlying these processes and generate innovative drugs for treating diseases.
To investigate PPIs in living mammalian cells, the mammalian two-hybrid assay (M2H)...