Protein-protein interactions play a critical role in virtually all cellular processes. Identifying and characterizing these interactions is therefore essential for understanding cellular signaling pathways, enzymatic reactions, and other biological processes. The yeast three-hybrid system (Y3H) is a powerful molecular tool for identifying and characterizing protein-protein...

Chemical cross-linking mass spectrometry (CXMS) is an important tool for resolving protein structures and studying protein interactions. The technique uses a chemical cross-linking agent to link two amino acids in close enough spatial proximity to each other in a covalent bond. The two amino acid sites where cross-linking occurs are identified by mass spectrometry. The distance between the...

Protein-protein interactions, commonly known as PPIs, are of utmost importance in various biological processes, including but not limited to signal transduction, gene expression, and metabolic pathways. The complexity and sheer significance of PPIs require us to comprehend them in their entirety to unravel the mechanisms underlying these processes and generate innovative drugs for treating...

The intricate interplay between DNA and histone proteins results in the formation of chromatin, which performs a pivotal function in governing the expression of genes. The dynamic process of chromatin remodeling and modifications plays a decisive role in regulating the accessibility of DNA to the transcriptional machinery, thereby leaving an indelible imprint on gene expression. Thus, the...